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rTamapin (K+ channel...
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rTamapin (K+ channel...

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Origin:Mesobuthus tamulus (Eastern Indian scorpion).

Source description:Recombinant,E.coli.

M.W.: 3459        Purity: >98%    

Effective concentration:10-100nM.

Sequence:AFCNLRRCELSCRSLGLLGKCIGEECKCVPY.

Modifications: Disulfide bonds between Cys3-Cys21, Cys8-Cys26, and Cys12-Cys28.

Molecular formula: C146H240N44O41S6.

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Sizes:50ug, 100ug, 5 x 50ug, 5 x 100ug lyophilized powder.

Activity:Tamapin blocks KCa2 channels, a highly potent blocker towards KCa2 channels.

Bioassay:

Tamapin inhibits SKCa3 channels heterologously expressed in HEK-293 cells.

 

SKCa3 channel currents, before and after application of 100nM Tamapin. The SKCa currents were elicited by 1µM internal calcium and 200 ms voltage ramps from -140 to +40 mV at the holding potential of 0 mV.

References:

1. Pedarzani, P. et al. (2002) J. Biol. Chem. 277, 46101.

2. Rodriguez de la Vega, R.C. and Possani, L.D. (2004) Toxicon 43, 865.

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