• 名称: rBeKm-1(K +通道毒素)
  • 编号: MPK-002
  • 浏览次数: 82
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Origin:Buthus eupeus (Lesser Asian scorpion).

Source description:Recombinant,E.coli.

M.W.:4092       Purity: >98%    

Effective concentration:2-10 nM.

Sequence:RPTDIKCSESYQCFPVCKSRFGKTNGRCVNGFCDCF.

Modifications: Disulfide bonds between Cys7-Cys28, Cys13-Cys33, and Cys17-Cys35.

Molecular formula: C174H267N51O52S6.

 

Sizes:50ug, 100ug, 5 x 50ug, 5 x 100ug lyophilized powder.

 

Activity:BeKm-1 inhibits ERG1 K+ channel currents and has minimal effect on ELK1 K+ channels.

Bioassay:

BeKm-1 inhibits Kv11.1 (hERG) channels expressed in HEK-293 cells.

 

hERG channel currents, before and after application of 1nM rBeKm-1. hERG channel currents were elicited by stepping the potential to −50 mVto +50 mV in 10 mV steps (1-s test potentials) from a holding potential of −80 mV for activation.

 

References:

1. Korolkova, Y.V. et al. (2001) J. Biol. Chem. 276, 9868.

2. Filippov, A.K. et al. (1996) FEBS Lett. 384, 277.