• 名称: rBmKTX(K +通道毒素)
  • 编号: MPK-003
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Origin:Mesobuthus Martensii (Buthus martensii).

Source description:Recombinant,E.coli.

M.W.:3962     Purity: >98%    

Effective concentration:0.1-1nM.

Sequence:VGINVKCKHSGQCLKPCKDAGMRFGKCINGKCDCTPK.

Modifications: Disulfide bonds between Cys7-Cys27, Cys13-Cys32, and Cys17-Cys34.

Molecular formula: C169H274N52O47S7.

 

Sizes:50ug, 100ug, 5 x 50ug, 5 x 100ug lyophilized powder.

 

Activity:

Voltage-gated channel (Kv1.3) blocker. rBmKTX blocks Kv1.3 channels with IC50 of 0.0019 nM.

Bioassay:

BmKTX inhibits Kv1.3 channels heterologously expressed in HEK-293 cells.

 

Kv1.3 channel currents, before and after application of 100pM BmKTX. Kv1.3 channel currents were elicited by depolarizing the voltage steps of 200 ms from the holding potential -80 mV to +50 mV.

 

References:

1. Romi-Lebrun R, et al. Biochemistry. (1997) 36:13473-82.

2.Renisio JG et al. Proteins. (2000) 38:70-8.

3. Dai L et al Biochem J. (2000) 346 Pt 3:805-9.