Enteropeptidase

  • Advanced Fermentation Platforms
  • Both Prokaryotic&Eukaryotic System
  • GMP-Compliant Pharmaceutical Purity
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Enteropeptidase

Recombinant Enteropeptidase (Enterokinase) is a high-purity, active, and specific enteropeptidase, it is produced by recombinant expression in Escherichia coli or Pichia pastoris. Recombinant Enterokinase (rEK) has the same featuring enzymatic cleavage sites as natural enterokinase, with cutting sites at Asp-Asp-Asp-Asp-Lys. This rEk can remove the fusion protein at the N-terminus, and eliminate unnecessary fusion tags.

In addition, rEK exhibits higher activity than the natural enteropeptidase, it can cleave fusion proteins effectively over a broad pH range of 4.5-9.5 and a wide temperature range. Even in various detergents and denaturing agents, it still retains partial activity.

Enteropeptidase
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Enteropeptidase Specification

  • Protease Name: Recombinant Enteropeptidase
  • CAS Number: 9014-74-8
  • Alternative Name: Enterokinase, EP, EK, Enteropeptidase, EC 3.4.21.9, Serine protease 7, ENTK, MGC133046
  • Grade: Bioactive, Animal Free, Carrier Free, GMP
  • Nature: Recombinant
  • Host Species:  E.coli
  • Source:  E.coli recombinant expression
  • Purity:  ≥95% (SDS-PAGE gel and HPLC analyses)
  • Endotoxin:  <1.0 EU per μg
  • Form:  Lyophilized powder
  • Appearance:  White, off-withe powder
  • Molecular Weight: 25.85kDa
  • Production Method: GMP Production
Trypsin Reference

Features

  • Specificity: rEK has specificity for the particular amino acid sequence, cleaves at the carboxyl-terminal site containing DDDDK(four aspartic acids with lysine).
  • Animal-free: recombinant methods ensure on contamination form exogenous viruses, this production process never involve any animal-derived materials.
  • High Purity: Devoid of other protease, ensure non-specific cleavage.
  • Consistent Quality: Stable and continuous supply with minimal lot-to-lot variation.
  • High Production Capacity: Morebio has fermentation systems ranging from 50L to 5000L, we are capable of diverse customer demands in different development and production stages.
  • Compliance with Regulations: All production facilities and environments adhere to relevant regulatory requirements. The manufacturing process adheres to GMP guidelines.
  • Comprehensive Quality Documentation: relevant regulatory support documents for customer requirements.

Application

  • Removal of N-terminal fusion tags, ensure accurate protein sequences
  • In biopharmaceutical field, production of GLP-1 analog peptide drugs
Enterokinase
Production Service

Enteropeptidase Production

Morebio develops our unique expression system of E.coli or Pichia pastoris for recombinant secretory proteins. We are the premier company to provide a professional and comprehensive microbial expression platform. We have years of experience with specific bioproducts in different microbial systems.

Custom Protein Expression

  • Large-scale production as requested
  • Tag removal as requested
  • Post-transitional modifications
  • Downstream purification system
  • Fast turn-around time
Custom Protein Expression

Gene Expression Workflow

  • Genes Optimization&Synthesis
  • Plasmid Construction
  • Transform & Induce
  • Protein Purification and Characterization
  • Post Processing
Gene Expression

Fermentation Capability

  • Identify and optimize the suitable expression system
  • Fermentation platform with large fermentation taks, bioreactors, large-scale purification systems
  • Genetic level for target enzymes production
  • High-purity and kilogram level products
Fermentation Capability

GMP Fermentation

  • GMP cell line banking with dedicated facilities
  • GMP manufacturing from pre-clinical to commercial scale
  • Validated GMP computer system
  • Advanced quality management systems
GMP Fermentation