Lys-C Protease

  • Advanced Fermentation Platforms
  • Both Prokaryotic&Eukaryotic System
  • GMP-Compliant Pharmaceutical Purity
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Lysine Endopeptidase

Lysine endopeptidase (Lys-C) is a serine protease, which is derived from Pseudomonas species. Lys-C can recognize lysine in proteins, and specially hydrolyze amide, ester, and peptide bonds on the carboxyl side of lysine.

Morebio provides recombinant lysine endopeptidase from express system of E.coli, it has the same specificity and activity as natural lysine endopeptidase. Our protease is free of other miscellaneous proteases, such as Lys-N, Arg-C, and serine proteases.

Lys-C
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Lys-C Specification

  • Protease Name: Lysyl Endopeptidase
  • CAS Number: 123175-82-6
  • Alternative Name: Lys-C, Achromobacter proteinase I, Achromobacter lyticus alkaline proteinase I, protease I, achromopeptidase, lysyl bond specific proteinase, and caseinase, Lep
  • Grade: Bioactive, Carrier Free, GMP
  • Nature: Recombinant
  • Host Species:  E.coli
  • Source:  E.coli recombinant expression
  • Purity:  ≥95% (SDS-PAGE gel and HPLC analyses)
  • Endotoxin:  <1.0 EU per μg
  • Form:  Lyophilized powder
  • Appearance:  White, off-withe powder
  • Molecular Weight: 29±3 kDa
  • Production Method: GMP Production
Lys-C Reference

Features

  • High Specificity: specific recognizance and cleavage of dibasic amino acids at the carboxyl end, Lys-Arg, Arg-Arg
    , Pro-Arg.
  • High Purity: ≥95%
  • Animal Free: Recombinant production, free of exogenous viral contamination, no animal-derived materials.

Application

  • Procession enzymatic cleavage in peptide drug production
  • Enzymatic digestion of proteins
  • Cleavage of fusion proteins
  • Peptide mapping, sequencing
  • Protein sequencing
Lysyl Endopeptidase
Production Service

Lys-C Production

Morebio develops our unique expression system of E.coli for recombinant secretory proteins. We are the premier company to provide a professional and comprehensive microbial expression platform. We have years of experience with specific bioproducts in different microbial systems.

Custom Protein Expression

  • Large-scale production as requested
  • Tag removal as requested
  • Post-transitional modifications
  • Downstream purification system
  • Fast turn-around time
Custom Protein Expression

Gene Expression Workflow

  • Genes Optimization&Synthesis
  • Plasmid Construction
  • Transform & Induce
  • Protein Purification and Characterization
  • Post Processing
Gene Expression

Fermentation Capability

  • Identify and optimize the suitable expression system
  • Fermentation platform with large fermentation taks, bioreactors, large-scale purification systems
  • Genetic level for target enzymes production
  • High-purity and kilogram level products
Fermentation Capability

GMP Fermentation

  • GMP cell line banking with dedicated facilities
  • GMP manufacturing from pre-clinical to commercial scale
  • Validated GMP computer system
  • Advanced quality management systems
GMP Fermentation