Kex2 Protease

  • Advanced Fermentation Platforms
  • Both Prokaryotic&Eukaryotic System
  • GMP-Compliant Pharmaceutical Purity
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Kex2 Protease

Kex2 Protease is also known as the double-alkaline endopeptidase and YSCF protease. It is a calcium-dependent serine protease responsible for processing the precursors of killer toxin and α-factor within yeast cells. Kex2 protease specifically recognizes and cleaves the peptide bonds of dibasic amino acids at the carboxyl end, like Lys-Arg, Arg-Arg, and Pro-Arg. Unlike Trypsin or Trypsinase, Kex2 cannot recognize and cleave the peptide bonds of single basic amino acids at the carboxyl end, such as arginine or lysine. Additionally, conventional serine protease inhibitors (PMSF, TPCK, TLCK) are ineffective in inhibiting the activity of Kex2 protease.

Morebio Recombinant Kex2 Protease is expressed in E.coli (Escherichia coli), and produced under GMP regulation. Our protease is free of any animal-derived components and without risk of viral contamination from animal sources.

Kex2
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Kex2 Specification

  • Protease Name: Recombinant Kex2
  • CAS Number: 99676-46-7
  • Alternative Name: QDS1, YNL238W, N1122, KEX2, Kexin, Protease KEX2, Proteinase YSCF
  • Grade: Bioactive, Carrier Free, GMP
  • Nature: Recombinant
  • Host Species:  E.coli
  • Source:  E.coli recombinant expression
  • Purity:  ≥95% (SDS-PAGE gel and HPLC analyses)
  • Endotoxin:  <1.0 EU per μg
  • Form:  Lyophilized powder
  • Appearance:  White, off-withe powder
  • Molecular Weight: 67.0±6.7 kDa
  • Production Method: GMP Production
Kex2 Reference

Features

  • High Specificity: specific recognizance and cleavage of dibasic amino acids at the carboxyl end, Lys-Arg, Arg-Arg
    , Pro-Arg.
  • High Purity: ≥95%
  • Animal Free: Recombinant production, free of exogenous viral contamination, no animal-derived materials.

Application

  • Procession enzymatic cleavage in peptide drug production
  • Enzymatic digestion of proteins
  • Cleavage of fusion proteins
  • Peptide mapping, sequencing
  • Protein sequencing
Kex2 Protease
Production Service

Kex2 Production

Morebio develops our unique expression system of E.coli for recombinant secretory proteins. We are the premier company to provide a professional and comprehensive microbial expression platform. We have years of experience with specific bioproducts in different microbial systems.

Custom Protein Expression

  • Large-scale production as requested
  • Tag removal as requested
  • Post-transitional modifications
  • Downstream purification system
  • Fast turn-around time
Custom Protein Expression

Gene Expression Workflow

  • Genes Optimization&Synthesis
  • Plasmid Construction
  • Transform & Induce
  • Protein Purification and Characterization
  • Post Processing
Gene Expression

Fermentation Capability

  • Identify and optimize the suitable expression system
  • Fermentation platform with large fermentation taks, bioreactors, large-scale purification systems
  • Genetic level for target enzymes production
  • High-purity and kilogram level products
Fermentation Capability

GMP Fermentation

  • GMP cell line banking with dedicated facilities
  • GMP manufacturing from pre-clinical to commercial scale
  • Validated GMP computer system
  • Advanced quality management systems
GMP Fermentation