PNGase F

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  • Both Prokaryotic&Eukaryotic System
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N-Glycosidase F

PNGase F is referred to as peptide N-glycosidase F. It is an amidase of the peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase class. PNGase F can cleave high mannose, hybrid, and complex oligosaccharide glycoproteins linked by asparagine residues from N-linked glycoproteins and glycopeptides. The cleavage site of PNGase F is the amide bond between the inner N-acetylglucosamine (GlcNAc) and asparagine residue of glycoproteins or glycopeptides, the asparagine on proteins will convert into aspartic acid after enzymatic hydrolysis.

PNGase F
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PNGase F Specification

  • Protease Name: PNGase F
  • CAS Number: 99676-46-7
  • Alternative Name: N-Glycosidase F; Peptide N-glycosidase F
  • Grade: Bioactive, Carrier Free, GMP
  • Nature: Recombinant
  • Host Species:  E.coli
  • Source:  E.coli recombinant expression
  • Purity:  ≥95% (SDS-PAGE gel and HPLC analyses)
  • Endotoxin:  <1.0 EU per μg
  • Form:  Lyophilized powder
  • Appearance:  White, off-withe powder
  • Molecular Weight: 36 kDa
  • Production Method: GMP Production
Kex2 Reference

Features

  • Fast: complete and rapid deglycosylation in a few minutes
  • High Purity: ≥95%, No protease, glycosidase contamination
  • Non Selective: Quickly remove all N-glycans without preference
  • Good Compatibility: can be applied in both used denaturing or no-denaturing conditions
  • Applied directly for downstream chromatography or MS spectrometry analysis

Application

  • Glycan Sequencing
  • Proteomics
  • Glycoprotein Analys
  • Recombinant Glycoprotein Expression
PNGase-F
Production Service

PNGase F Production

Morebio develops our unique expression system of E.coli for recombinant secretory proteins. We are the premier company to provide a professional and comprehensive microbial expression platform. We have years of experience with specific bioproducts in different microbial systems.

Custom Protein Expression

  • Large-scale production as requested
  • Tag removal as requested
  • Post-transitional modifications
  • Downstream purification system
  • Fast turn-around time
Custom Protein Expression

Gene Expression Workflow

  • Genes Optimization&Synthesis
  • Plasmid Construction
  • Transform & Induce
  • Protein Purification and Characterization
  • Post Processing
Gene Expression

Fermentation Capability

  • Identify and optimize the suitable expression system at genetic level for target enzymes production
  • Fermentation platform with large fermentation taks, bioreactors, large-scale purification systems
  • High-purity and kilogram level products
Fermentation Capability

GMP Fermentation

  • GMP cell line banking with dedicated facilities
  • GMP manufacturing from pre-clinical to commercial scale
  • Validated GMP computer system
  • Advanced quality management systems
GMP Fermentation